For denv 1 and denv 2, five genotypes have been described. The four denv serotypes can cause a wide range of diseases in humans. Onestep realtime rtpcr assays for serotyping dengue virus. In this study, we used af denv serotype 1 af denv 1 together with af denv 2 on peripheral blood mononuclear cells pbmcs from children in thailand with acute primary or secondary denv 1 infections to analyze the phenotypes of antigenspecific b cells that reflected their exposure or clinical diagnosis. The dengue virus molecular typing method described by lanciotti and coworkers r. New dengue virus type 1 genotype in colombo, sri lanka. This study revealed that serotype diversity among the three dengue fever endemic areas in south sulawesi did occur.
However, it failed to detect denv 1 variants in cambodia due to a point mutation. In humans, dengue infection causes a spectrum of illnesses ranging from asymptomatic, fever, rash, joint pain and other mild symptoms to lifethreatening. Denv 3 and denv 4 have been subdivided into 4 and 2 genotypes, respectively 3,4. The various steps in the flavivirus life cycle include 1 virions binding to. Dengue virus denv is the arbovirus with the highest incidence in new caledonia and in the south pacific region. Considering that the vector mosquito, aedes albopictus, already exists in south korea, we propose that a vector surveillance program for dengue is urgently needed.
Assessment of a dengue1viruslive virus human challenge denv 1lvhc virus strain the safety and scientific validity of this study is the responsibility of the study sponsor and investigators. In 20122014, a major denv1 outbreak occurred in new caledonia. Clinical manifestations of dengue in relation to dengue. Df is distributed in most tropical and subtropical areas, where aedes aegypti andor a. Denv 5 has been detected during screening of viral samples taken from a 37 year old farmer admitted in hospital in sarawak state of malaysia in the year 2007. The only known vector of denv in new caledonia is aedes aegypti but no study has yet evaluated the competence of new caledonia ae.
The serotypes of dengue virus in endemic areas of makassar, gowa, and maros were denv 2 and denv 4 and no denv 1 and denv 3 serotypes were found. Clinical significance of the findings are that most of the people living in delhi are probably immune to denv 3 and denv 1 but chances of an outbreak by less prevalent serotypes are still there. The four dengue virus denv serotypes, denv 1, 2, 3, and 4, are endemic throughout tropical and subtropical regions of the world, with an estimated 390 million acute infections annually. Subsequent infection with different serotype of the virus leads. Dengue virus has four serotypes, namely, denv1, denv2, denv3, and denv4. Infection with a single serotype leads to longterm protective immunity against the homologous serotype but not against other serotypes 1. Epidemiological and molecular features of dengue virus type1. We successfully isolated seven denv serotype1 strains for the first. In the last decades, the global distribution of dengue virus denv has expanded to include more geographic areas and all four serotypes denv14 are now present in asia, africa, and the americas. Dengue is the most widespread mosquitoborne viral disease with an estimated 390 million infections yearly 1, 2. Majority of the dengue outbreaks are attributed to denv 2 and denv 3 viruses, but denv 1 has not been recorded in major dengue outbreaks. Dengue virus 3 genotype 1 associated with dengue fever and. Phylogenetic analysis revealed that strain ynpe1 belonged to genotype i. Silent circulation of dengue virus in aedes albopictus.
Overlapping nucleic acid sequences were combined for analysis and edited with the aid of sequencher software gene code corp. Dengue virus serotypes 1 and 2 responsible for major dengue outbreaks in nepal. Listing a study does not mean it has been evaluated by the u. Dengue is a febrile illness transmitted by mosquitoes, causing disease across the tropical and subtropical world. Nonetheless, these results show that all four serotypes have been widely circulating in most of indonesia, as is common in hyperendemic countries. Identification of dengue type 1 virus denv1 in koreans. After recovering from an infection with one dengue serotype, a person has immunity against that particular serotype.
Complete genome sequence analysis of an imported dengue virus. All the isolates were identified as denv serotype 1. Why is the discovery of the fifth serotype of dengue denv. May 14, 2019 epidemiology investigation and phylogenetic analysis of circulating viruses revealed that at least three lineages of dengue virus serotype 2 denv 2 cosmopolitan genotype initiated the outbreak. Sequencing and phylogeny of tn dhole dengue virus 1. The ec 50 values of niclosamide against denv1, denv2, denv3, and denv4 were approximately 1. Diversity of dengue virus serotype in endemic region of. Antibody prevalence data and serotype distribution describe populationlevel risk and inform public health decisionmaking. We report the fullgenome sequence of a dengue serotype 1 virus denv 1 isolated from a traveler returning in july 2019 to italy from democratic republic of congo drc, which is currently affected by ebola and measles outbreaks. Assessment of a dengue1viruslive virus human challenge.
Dengue virus and its four serotypes denv 1 to denv 4 infect 390 million people and are implicated in at least 25,000 deaths annually, with the largest disease burden in tropical and subtropical regions. The proportion of children with exposure to 1 serotype increased with age, and children were more likely to have been infected with denv 2, denv 1 and denv 3 than denv 4. Denv1, 2, and 3 were recovered in approximately similar ratios each year table 2. While there are commercially available elisa assays for denv ns1 detection, these tests have limited sensitivity 5070%, do not determine the serotype of the infecting denv, do not detect all four serotypes equally, or are less sensitive in subsequent denv infections. In 1998 and 1999, all four dengue virus serotypes denv14 were recovered from patients in the yangon childrens hospital.
Natural history of dengue virus denv1 and denv4 infections. Here, we report the first complete genome sequence and molecular characterization of the imported dengue virus serotype 1 strain ynpe1. Denv 2 was mainly detected on both occasions and this serotype caused nearly 95% of denv infections. In 2000 and 2001, no denv4 was recovered, and denv1 was recovered more frequently than any other serotype. The first encounter with a dengue virus strain is not often fatal and results in immunity of that strain but only limited or temporary crossimmunity against other strains. Clade replacements in dengue virus serotypes 1 and 3 are. Rapid displacement of dengue virus type 1 by type 4, pacific. All statistical data were analyzed using the statistical software jmp in. Denv 1 variant strains that escape diagnosis by virus isolation and identification with the typespecific monoclonal antibody 15f3 1 15 reacting with ns1 protein instead of the monoclonal antibody d21f reacting with the e protein 6, 11 have been described to our knowledge, ours is the first description of falsenegative results due to a genetic variant. Phylogenetic and molecular analyses showed extensive variability among the denv serotypes, which led to the recognition of different genotypes within each serotype. We describe an improvement of the method that allows the. In the following proofofconcept study, we demonstrate the use of the atcc synthetic denv molecular standards in the development of a standard curve for the quantification of viral rna extracted from representative samples of all four denv serotypes. The database was established by excel software, the figures were. What is different between dengue virus serotypes den1,den2,den3 and den4.
The fifth and latest addition to the existing serotypes of dengue viruses is denv 5 which has been announced in october 20. Denv 3, which has been found to be the predominant dengue virus circulating to predominant dengue virus serotype circulating in northern india 6,7,8. Clinical, laboratory, and epidemiological features. The existing trend contended that serotype replacement, most probably with denv 3 is predicted to take place once the current outbreak subsides. Denv 1 caused a small number of infections in the 2010 outbreak. Although sporadic imported cases of denv have been documented in yunnan, china since 2000, a complete genome sequence of dengue virus serotype 3 denv 3 imported from laos is still not available. Neutralization of acidic intracellular vesicles by. Here, we report the first complete genome sequence and genomic characterization of a denv 3. Seqman program in the lasergene software version 5. Why is the discovery of the fifth serotype of dengue denv 5 not published yet. Diversity of dengue virus serotype in endemic region of south. For invitro diagnostic use ivd performance characteristics have been determined. There are four serotypes of the dengue virus denv 1, denv 2, denv 3 and denv 4 differing by 3035% in amino acid sequence.
Jul 24, 2018 dengue virus denv infection caused by international visitors has become a public health concern in china. Reemergence of dengue virus serotype 3 infections in gabon. Monoclonal antibodies for specific detection of dengue virus. Serotype of dengue virus has correlation with severity of dengue fever due to host immune response. Pdf dengue virus serotypes 1 and 2 responsible for major. Denv 1 strain predominated the circulation in the nonepidemic period 5. The further classification of surabaya denv1 into two different genotypes warrants indepth genomic analysis to study the dynamics of both genotypes and their contribution to virus evolution, virus transmission, and disease.
Dengue virus 3 genotype 1 associated with dengue fever and dengue hemorrhagic fever, brazil. How do dengue viruses infect human cells and replicate themselves. Denv1 through denv4 are members of the flavivirus group of the virus. Mar 31, 2014 the occurrence of two consecutive outbreaks due to the same dengue serotype had historically already been observed in nc and other picts7, 11. Dengue virus denv is a singlestranded, positivesense rna virus belonging to the genus flavivirus, family flaviviridae. This indicated that denv 1 had replaced a formerly predominant denv 2. Freezedried dengue virus 1, strain uve denv 1 20do94, dominican republic, 20, complete genome. Immunity will form when a person is infected with one type of serotype but does not apply when a secondary infection develops with another serotype. We performed fulllength genome sequencing to nine isolates representatives from denv1 genotype i and genotype iv. Multiple lineages of dengue virus serotype 2 cosmopolitan. Apr 01, 2007 dengue fever df is a vectorborne disease caused by 4 closely related dengue viruses denv 14 1, 2.
Dengue virus denv is a flavivirus transmitted by aedes spp. Molecular characterization and phylogenetic analysis of a. Conclusionwe successfully isolated seven denv serotype 1 strains for the first time in south korea from imported infections. The development of a novel serotypingns1elisa to identify serotypes of dengue virus chunya puttikhunt a,b. The four antigenically distinct serotypes denv 1, 2, 3, and 4 cause various forms of illness, ranging from inapparent infection or classic dengue fever df to severe and lifethreatening dengue hemorrhagic feverdengue shock syndrome. It has been determined that recent dengue virus epidemics in yunnan, china, originated from southeast asian strains. Dengue virus type 1 denv1 is one of the four serotypes of the. The etiological agent of the disease is an arbovirus of the genus flavivirus and family flaviviridae with four antigenically distinct serotypes denv 1, denv 2, denv 3 and denv 4 1,3,4 which is. We investigated the spatial dynamics of denv 1, denv 2 and denv 3 in brazil by applying a statistical framework to complete genome sequences. Denv1 classification of the brun2014 strain was further supported. Infection with 1 denv serotype is believed to provide longterm immunity to the homologous serotype but not to the other serotypes 2. In the present study, denv 1 was primarily present at the study area from 20142017. There are four dengue virus serotypes, denv 1 4, which are genetically and antigenically distinct, although each serotype elicits a similar range of disease manifestations during infection.
Highly divergent dengue virus type 1 genotype sets a new distance. Circulation of dengue virus1 denv1 serotype in delhi. The development of a novel serotypingns1elisa to identify. Atcc development and use of synthetic molecular standards. Why is the discovery of the fifth serotype of dengue denv5 not published yet. Five serotypes of the virus have been found, all of which can cause the full spectrum of disease. Improved molecular detection of dengue virus serotype 1. Dengue virus serotype 1 vlp the native antigen company. The natural history of wildtype dengue virus denv infections of humans. Dengue virus denv nonstructural protein 1 ns1 is secreted in blood during the acute phase of viremic denv infection. Dengue virus serotype 1 vlp viruslike particle is a new, unique product that has been developed in response to the need for highly immunogenic, concentrated dengue antigens for use in dengue focussed disease research and vaccine development including use as an immunogen. Dengue virus denvns1 serotype 1 antigen, recombinant. It is a mosquitoborne, single positivestranded rna virus of the family flaviviridae. Why is the discovery of the fifth serotype of dengue denv5.
Dengue virus envelope protein domain iii hinge determines. Pcr assay is a nucleic acid amplificationassay that detects denv serotypes 1,2, 3 or 4 rna from human serum or plasma collected from human patients with signs and symptoms consistent with dengue infection. Freezedried dengue virus 1, strain uvedenv120do94, dominican republic, 20, complete genome. Infection with a single serotype leads to longterm protective immunity against the homologous serotype but not against other serotypes. The etiological agent of the disease is an arbovirus of the genus flavivirus and family flaviviridae with four antigenically distinct serotypes denv1, denv2, denv3. Cdc denv 1 4 realtime rtpcr assay for detection and serotype identification of dengue virus instructions for use package insert catalog no. Thus, persons can be infected with multiple serotypes during their lifetime. Dynamics of dengue virus denvspecific b cells in the. During 19972000 in the pacific region, the serotype recovered from patients was almost exclusively denv2, but during 20002001, virus denv from patients with suspected dengue fever is important both for the diagnosis of the disease and for the implementation of epidemiologic control measures. What is different between dengue virus serotypes den1. In contrast, denv 3 has only been detected in one individual in gabon so far caron et al. Differences in the transmission of dengue fever by different. A technique for the multiplex detection and typing of denv serotypes 1 to 4 denv 1 to denv 4 from clinical samples by pcrligase detection reaction ldr has been developed. Dengue virus serotype distribution based on serological.
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